Diagnostic Testing

A $10 submission fee and 1% hazardous fee is assessed on each case.

Testing Schedule

Our goal is to provide accurate results and timely service. However, many research and diagnostic assays are not performed daily and/or testing schedules may change. If your case involves testing animals for export, show animals, animals to be sold, and/or a large number of samples, please contact the VDL (515-294-1950) prior to submission to verify the availability and turnaround time.

Fees and test schedules are subject to change without notice.
For additional test information, please click the pathogen name.

* Preferred specimen for testing.
^ Testing performed in part or in total at a Referral Laboratory.
^^ Assay has not been fully validated for all the testing conducted.

Pathogen	Species	Test	Section	Standard Fee	Best Practice Fee	Days Tested	Turn Around	Specimen	Comments	File Attachment
	All Species	Digital Imaging - Slide Scan	Histopathology	$15.00					15.00/scan Please contact the coordinating pathologist, laboratory manager or digital technician for requests and questions regarding this service. Image transfer will be to user or lab provided flash drive (additional fee if lab provided).
Actinobacillus pleuropneumoniae - APP	Porcine	PCR - serotyping	Molecular	$60.00	$54.00	M, Th	next day	culture	detects serotypes 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 15
Actinobacillus pleuropneumoniae - APP	Porcine	CF 1, 5, 7 (no serotyping)	Serology	$5.00	$4.50	W	2-day test	serum
Actinobacillus pleuropneumoniae - APP	Porcine	Mix APP ELISA	Serology	$12.00	$10.80	F	same day	serum	MIX APP ELISA generates 3 results for these serotype groups: 1-2-9-11, 3-6-8-15 and 4-5-7
Actinobacillus pleuropneumoniae - APP	Porcine	PCR - screen	Molecular	$30.00	$27.00	M, Th	next day	culture, lung, oral fluid
Actinobacillus pleuropneumoniae - APP	Porcine	ApxIV ELISA	Serology	$6.00	$5.40	W	same day	Serum, plasma	Detects antibody against the ApxIV toxin produced by some Actinobacillus pleuropneumoniae. DOES NOT DETECT SEROTYPE. Useful for population surveillance.
Actinobacillus pleuropneumoniae - APP	Porcine	CF Serotype 1, 5, 7	Serology	$15.00	$13.50	W	2-day test	serum
Actinobacillus pleuropneumoniae - APP	Porcine	CF Serotype 3	Serology	$5.00	$4.50	W	2-day test	serum
Actinobacillus pleuropneumoniae - APP	Porcine	ELISA 10-12	Serology	$4.00	$3.60	F	same day	serum
Actinobacillus suis	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	lung, bronchial swab, culture, laryngeal swab, oral fluid, pharyngeal swab
Ammonia	All Species	HPLC	Analytical Chemistry Services	$30.00			5-7 days	*rumen contents, *ocular fluid (aqueous or vitrous humor), serum/plasma or urine	Frozen (1-2 drops of saturated Mercuric Chloride (HgCl2) may be used instead of freezing rumen contents)
Anaplasma marginale	Bovine	cELISA	Serology	$6.00	$5.40	T, Th	same day	serum
Anaplasma marginale	Bovine, Caprine, Equine, Ovine	PCR	Molecular	$35.00	$31.50	M, Th	next day	whole blood (EDTA), spleen
Anaplasma marginale & phagocytophilum	Bovine, Caprine, Equine, Ovine	PCR	Molecular	$40.00	$36.00	M, Th	next day	Whole blood, spleen	This test detects and differentiates Anaplasma marginale and Anaplasma phagocytophilum
Antibiotic Panel - Feed	All Species	LC-MS	Analytical Chemistry Services	$150.00		Tu	3-5 days	*Corn-based Feed, DDGS, grain, mixed feed, silage, total mixed ration, mineral premixes (200g)	Panel includes: Bacitracin, Carbadox, Chlortetracycline, Florfenicol, Lincomycin, Oxytetracycline, Penicillin, Tetracycline, Sulfadimethoxine Sulfamethazine, Sulfathiazole, Tiamulin, Tilmicosin, Tylosin, Tylvalosin, Virginiamycin. Individual antibiotics in the panel can be quantified separately for additional fee. Expected concentration required upon submission. If expected concentration is not given upon submission then additional charges may apply. STAT fees may apply (minimum 2x the normal fee).
Antibiotics Panel - Milk	Bovine	LC-MS	Analytical Chemistry Services	$100.00		W	1-3 days	Milk	Panel includes: Amoxicillin, Ampicillin, Ceftiofur, Cephapirin, Desacetylcephapirin, Cloxacillin, Dicloxacillin, Oxacillin, and Penicillin G. Individual antibiotics in the panel in addition to a variety of other veterinary drugs can be quantified separately for an additional fee. STAT fees may apply (minimum 2x the normal fee). All samples should be frozen and maintained frozen during shipment.
Anticoagulant Panel	All Species	LC-MS	Analytical Chemistry Services	$95.00			5-7 days	*whole blood-EDTA (4mL), *liver (10g)	Panel Includes: Brodifacoum, Bromodiolone, Chlorophacinone, Coumachlor, Dicoumarol, Difenacoum, Difethialone, Diphacinone, and Warfarin
Atypical Porcine Pestivirus - APPV (Congenital Tremor)	Porcine	PCR	Molecular	$30.00	$27.00	M-F	same day	serum, brain, fecal swab, nasal swab, heart		more info
Avian adenovirus group 1 (types D and E) - AvAdV-1	Avian	PCR	Molecular	$35.00	$31.50	M, Th	Next day	Cloacal swab, tracheal swab
Avian encephalomyelitis virus - AEV	Avian	ELISA	Serology	$2.75	$2.48	W	same day	serum	validated for chicken only
Avian hemorrhagic enteritis virus - HEV	Avian	ELISA	Serology	$2.50	$2.25	M	Same day	Serum	Turkey only
Avian hepatitis E virus - AvHepEV	Avian	PCR	Molecular	$45.00	$40.50	M, Th	next day	liver, feces, fecal swab, intestine
Avian infectious bronchitis virus - AvIBV	Avian	ELISA	Serology	$2.75	$2.48	Tuesday	same day	serum	validated for chicken only
Avian infectious bronchitis virus - AvIBV	Avian	PCR	Molecular	$30.00	$27.00	M, Th	Same day	Lungs, trachea
Avian influenza virus - AIV	Avian	ELISA	Serology	$3.00	$2.70	M-F	same day	serum	Avian species - chicken, turkey, goose, duck and ostrich
Avian influenza virus - AIV	Avian	AGID - serum	Serology	$3.00	$2.70	M-F	2-day test	serum	Testing performed on Friday will have results available the following Monday
Avian influenza virus - AIV	Avian	AGID - egg yolk	Serology	$3.50	$3.15	M-W, F	2-day test	egg/yolk	Testing performed on Friday will have results available the following Monday
Avian influenza virus - AIV	Avian	PCR	Molecular	$30.00	$27.00	M-F	same day	cloacal swab, oropharyngeal swab, tissue samples, drinker swab*	Drinker swab (or environmental water swab) testing is available, but only for submitter-funded surveillance testing.
Avian influenza virus - AIV	Avian	VI	Virology	$50.00		M, Th	7-21 days	cloacal or oropharyngeal swab, feces, oral+cloacal, tracheal swab, environmental sample
Avian influenza virus - AIV	Avian	PCR - H5	Molecular	$30.00	$27.00	M-F	same day	cloacal swab, oropharyngeal swab, tissue samples
Avian metapneumovirus - aMPV	Avian	ELISA	Serology	$2.50	$2.25	Th	same day	serum
Avian paramyxovirus-1 - aPMV-1 (NDV)	Avian	VI	Virology	$50.00		M, Th	7-21 days	cloacal swab, oropharyngeal swab, feces, tracheal swab, environmental
Avian paramyxovirus-1 matrix gene - aPMV-1 (NDV)	Avian	PCR	Molecular	$30.00	$27.00	M-F	same day	cloacal swab, oropharyngeal swab, allantoic fluid, bronchial swab, pharyngeal swab, tracheal swab
Avian reovirus - AvRV	Avian	ELISA	Serology	$2.50	$2.25	W	same day	serum
Avian reovirus - AvRV	Avian	PCR	Molecular	$30.00	$27.00	M, Th	Next day	tendons
Avibacterium paragallinarum - AvPGal	Avian	PCR	Molecular	$40.00	$36.00	M, Th	next day	Oropharyngeal swab, tracheal swab, lung
AvILTV	Avian	PCR	Molecular	$30.00	$27.00	M, Th	Same day	Lungs, trachea
Bacterial Basic Quantification	All Species	Basic Quantification	Bacteriology	$10.00		M-F	2-5 days	Urine (voided, catheterized, clean catch or midstream, or cage floor), semen, prostate wash/fluid	Fee is per sample and in addition to routine culture. This test only quantifies the bacteria present directly from the sample in 0.1 mL, 0.01 mL, and 0.001 mL with no dilution.
Bacterial isolation and identification - Swabs	Avian, Bovine, Caprine, Ovine, Porcine, Ungulates	Culture	Bacteriology	$15.00		M-F	3 - 7 days	Swab	Vaginal swabs are $25 each. Porcine nasal swabs are only effective for culturing Pasteurella and Bordetella. Samples with inherently high level of background flora (e.g., fecal samples of any species, swine nasal/oral fluid samples), are not pooled.
Bacterial isolation and identification - Swabs	Camelid, Canine, Cervidae, Equine, Feline, Rodent	Culture	Bacteriology	$25.00		M-F	3 - 7 days	Swab
Bacterial isolation and identification - Tissue Individual	All Species	Culture	Bacteriology	$25.00		M-F	3 - 7 days	Tissue	$25.00 1st sample, $10.00 for each additional sample. For diagnostic submissions where individual testing on animals from same epidemiological unit (age, group, or lot) is requested. Large submissions may incur additional fees.
Bacterial isolation and identification - Tissue Routine	All Species	Culture	Bacteriology	$25.00		M-F	3 - 7 days	Tissue	$25.00 1st sample; $7.50 for each additional sample. For diagnostic submissions where individual testing is not requested. Large submissions may incur additional fees.
Bacterial susceptibility testing	All Species	Susceptibility	Bacteriology	$25.00		M-F	1-5 days		Includes aerobic, anaerobic, and microaerophilic organisms
Baculovirus	All Species	PCR - Takara Bio Baculovirus Quantitation	Research	$250.00		as needed		Isolate	This assay is for quantitating baculoviral stocks generated from AcMNPV-based baculoviral vectors using the Takara Bio BacPAK qPCR kit. $250 for first sample, $50 for each subsequent sample
Biopsy	All Species	Biopsy	Histopathology	$75.00		as needed			Up to 5 slides per animal (each additional slide $7)
Block preparation & return	All Species	Block preparation	Histopathology	$5.50		as needed			Paraffin-embedded block preparation & return only
Blood Culture	All Species	Bacterial Isolation and Identification	Bacteriology	$30.00		M-F	3 - 6 days	Blood culture bottle (aerobic and/or anaerobic)	Samples must be submitted in commercial blood culture bottles
Bluetongue - BTV	Bovine, Caprine, Cervidae, Ovine, Ungulates	cELISA	Serology	$8.00	$7.20	M	Same day	serum
Bluetongue and Epizootic Hemorrhagic Disease - BTV and EHD	Bovine, Camelid, Caprine, Cervidae, Ovine, Ungulates	AGID	Serology	$7.50	$6.75	M-Th	next day	serum
Bluetongue virus - BTV	Bovine, Caprine, Cervidae, Ovine, Ungulates	PCR	Molecular	$35.00	$31.50	M, Th	next day	whole blood, spleen
Bone Profile	All Species		Analytical Chemistry Services	$75.00		F	1-3 days	*whole 2nd rib	Price: $75 ($50 each for 5 or more bones submitted) Includes: ash, density, calcium, phosphorus
Bordetella avium	Avian	PCR	Molecular	$40.00	$36.00	M, Th	next day	oropharyngeal swab, tracheal swab, lung
Bordetella avium E	Avian	ELISA	Serology	$2.50	$2.25	M	Same day	Serum	Turkey only
Bovine abortion panel - BAP	Bovine	PCR	Molecular	$55.00	$49.50	M-F	Same day	Fetal Tissues	Fetal tissues should include ALL of the following: kidney, liver, lung, placenta, skeletal muscle, and spleen. Includes Bovine herpesvirus-1, Bovine viral diarrhea virus, Leptospira species, and Neospora caninum.
Bovine coronavirus - BCoV	Bovine, Caprine, Ovine	IHC	Histopathology	$25.00		as needed		tissue
Bovine coronavirus - BCoV	Bovine	PCR	Molecular	$30.00	$27.00	M-F	same day	intestine, feces, fecal swab
Bovine enteric panel - BEP	Bovine, Caprine, Ovine	PCR	Molecular	$55.00	$49.50	M-F	same day	feces, intestinal content, fecal swab, intestine	Includes Rotavirus group A, BCV, Salmonella spp, E coli K99, Cryptosporidium parvum
Bovine herpesvirus - BHV-1	Bovine	PCR	Molecular	$30.00	$27.00	M-F	next day	lung, nasal swab
Bovine herpesvirus - BHV-1	Bovine	VN	Virology	$5.00		F	following Monday	serum
Bovine herpesvirus - BHV-1	Bovine	VI	Virology	$35.00		as needed	7-14 days	lung, nasal swab, aborted fetal tissue
Bovine herpesvirus - BHV-1	Bovine, Caprine, Ovine	IHC	Histopathology	$25.00		as needed		tissue
Bovine leukemia virus - BLV	Bovine, Ungulates	ELISA	Serology	$5.00	$4.50	T, Th	same day	serum	Bison
Bovine parainfluenza virus type 3 - BPIV-3	Bovine	VN	Virology	$5.00		F	M	serum
Bovine parainfluenza virus type 3 - BPIV-3	Bovine	PCR	Molecular	$30.00	$27.00	M, Th	next day	BAL, lung, nasal swab, tracheal wash
Bovine parainfluenza virus type 3 - BPIV-3	Bovine	VI	Virology	$35.00		as needed	7-14 days	lung, nasal swab
Bovine respiratory panel bacterial - BRP Bacti	Bovine, Caprine, Ovine	PCR	Molecular	$40.00	$36.00	M-F	same day	lung, pharyngeal swab, tracheal wash, BAL	Includes Mycoplasma bovis, Mannheimia haemolytica, Histophilus somni, Pasteurella multocida
Bovine respiratory panel viral - BRP Viral	Bovine, Caprine, Ovine	PCR	Molecular	$40.00	$36.00	M-F	same day	lung, nasopharyngeal swab, TTW, BAL	Includes Bovine Coronavirus, Bovine Herpesvirus type 1, Bovine Respiratory Syncytial Virus, and Bovine Viral Diarrhea Virus
Bovine respiratory panel viral and bacterial - BRP	Bovine, Caprine, Ovine	PCR	Molecular	$80.00	$72.00	M-F	same day	lung, nasal swab, pharyngeal swab, tracheal wash, BAL	Includes Bovine Coronavirus, Bovine Herpesvirus type 1, Bovine Respiratory Syncytial Virus, Bovine Viral Diarrhea Virus, Mycoplasma bovis, Mannheimia haemolytica, histophilus somni, Pasteurella multocida
Bovine respiratory syncytial virus - BRSV	Bovine, Caprine, Ovine	IHC	Histopathology	$25.00		as needed		lung
Bovine respiratory syncytial virus - BRSV	Bovine, Caprine, Ovine	PCR	Molecular	$30.00	$27.00	M-F	same day	lung, nasal swab, pharyngeal swab, tracheal wash, BAL
Bovine respiratory syncytial virus - BRSV	Bovine	VN	Virology	$5.00		F	following Friday	serum
Bovine respiratory syncytial virus - BRSV	Bovine	VI	Virology	$35.00		as needed	one week	Lung, nasal swab, oropharyngeal swab
Bovine viral diarrhea virus - BVDV	Bovine	AgELISA	Serology	$6.00	$5.40	W, F	same day	serum, ear notches (fresh, not fixed)
Bovine viral diarrhea virus - BVDV	Bovine, Caprine, Ovine	PCR - screen	Molecular	$30.00	$27.00	M-F	same day	feces, intestinal content, fecal swab, buffy coat, intestine, lung, lymph node, semen, serum, spleen, milk, fresh ear notch	Turn around time is NEXT DAY for fresh ear notches and EDTA whole blood buffy coat samples.
Bovine viral diarrhea virus - BVDV	Bovine	IHC (1-3 notches)	Histopathology	$20.00	$18.00	M-F	next day	formalin-fixed ear notches	For surveillance cases only.
Bovine viral diarrhea virus - BVDV	Bovine	VI	Virology	$35.00		M, W	7-10 days	blood, lung, serum, semen
Bovine viral diarrhea virus - BVDV	Bovine	VN- type I	Virology	$5.00		F	following Wednesday	serum
Bovine viral diarrhea virus - BVDV	Bovine	VN- type II	Virology	$5.00		F	following Wednesday	serum
Bovine viral diarrhea virus - BVDV	Bovine	IHC (4 or more notches)	Histopathology	$3.75	$3.38	M-F	next day	formalin-fixed ear notches	$3.75 per notch. For surveillance cases only.
Bovine viral diarrhea virus - BVDV	Bovine	IHC	Histopathology	$25.00		as needed		lung, spleen, thymus	For diagnostic cases only
Bovine Viral Diarrhea Virus and Bovine Herpesvirus type 1 - BVDV/BHV-1	Bovine, Caprine, Ovine	PCR	Molecular	$35.00	$31.50	M-F	same day	lung, nasopharyngeal swab, BAL
Brachyspira	Avian, Canine, Porcine	Culture - Routine	Bacteriology	$20.00		M-F	1-2 weeks	colon, feces, or fecal swabs	This test is intended to screen for strongly beta-hemolytic and weakly beta-hemolytic Brachyspira species. Due to the inherently high level of background flora in fecal samples, the ISUVDL Bacteriology Section does not pool samples for Brachyspira culture. Samples are set up individually, reported individually, and charged individually.
Brachyspira	Porcine	Culture - Dysentery Surveillance	Bacteriology	$15.00		M-F	1-2 weeks	Colon, feces, or fecal swabs	This test is intended to screen for strongly beta-hemolytic Brachyspira species only. If weakly beta-hemolytic Brachyspira species are of clinical concern, please select Routine Brachyspira Culture. Due to the inherently high level of background flora in fecal samples, the ISUVDL Bacteriology Section does not pool samples for Brachyspira culture. Samples are set up individually, reported individually, and charged individually.
Brachyspira	Avian, Bovine, Canine, Porcine	Susceptibility	Bacteriology	$40.00		M-Th	1 - 2 weeks	feces, colon contents, oral fluids
Brachyspira hyodysenteriae/hampsonii	Porcine	PCR	Molecular	$45.00	$40.50	M, Th	next day	feces, fecal swab, culture	Duplex real-time PCR intended for diagnosing swine dysentery (SD) associated with Brachyspira hyodysenteriae or Brachyspira hampsonii infections in swine.
Brachyspira spp.	Porcine	Sequencing (nox gene)	Molecular	$50.00	$45.00	as needed	2-3 days	identification from culture
Brucella	Bovine, Canine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates	B. abortus/suis BAPA Screen	Serology	$1.00	$0.90	M-F	same day	serum	Bison
Brucella	Bovine, Camelid, Canine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates	B. abortus/suis/melitensis Card	Serology	$2.00	$1.80	M-F	same day	serum, plasma	Unless specified by export, all swine sera submitted for the Card test will be tested for Brucellosis on the BAPA
Brucella	Bovine, Porcine	B. abortus FPA	Serology	$7.50	$6.75	M-F	same day	serum
Brucella	Bovine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates	Bruc. SPT	Serology	$2.50	$2.25	M-F	same day	serum	Bison
Brucella	Bovine, Equine, Ovine, Porcine	Bruc. STT	Serology	$2.50	$2.25	M-W	3-day test	serum
Brucella canis	Canine	B. canis Rapid Ab	Serology	$20.00	$18.00	M-F	same day	whole blood, plasma, and serum	If positive will be automatically tested on B. canis TAT
Brucella canis	Canine	B. canis TAT	Serology	$15.00	$13.50	M-W	48 hours	serum
Bulk Tank Milk	Bovine	Culture	Bacteriology	$35.00		M-Th	4 - 6 days	bulk tank milk	Mycoplasma culture may also be requested with this test
Campylobacter	All Species	Culture	Bacteriology	$10.00		M-F	1 week		Samples should be collected in transport media such as Weybridge and arrive at the laboratory on Monday-Friday during business hours within 24-48 hours of collection for test validity. Refrigerated or frozen samples are not suitable for culture.
Campylobacter jejuni and fetus	Bovine, Caprine, Ovine	PCR	Molecular	$35.00	$31.50	Daily	Same day	Placenta, fetal tissues, stomach content
Canine adenovirus - CAdV	Canine	PCR	Molecular	$40.00	$36.00	M, Th	next day	liver, laryngeal swab, tracheal swab, lung, nasal swab
Canine adenovirus - CAdV	Canine	VI	Virology	$35.00		as needed	7-14 days	lung, feces, intestine, nasal swab
Canine coronavirus - CCoV	Canine	PCR	Molecular	$40.00	$36.00	M, Th	next day	feces, fecal swab
Canine distemper virus - CDV	Canine	PCR	Molecular	$40.00	$36.00	M, Th	next day	lung, brain, nasal swab
Canine herpesvirus - CHV	Canine	PCR	Molecular	$40.00	$36.00	M, Th	next day	lung, nasal swab, kidney, liver
Canine herpesvirus - CHV	Canine	VI	Virology	$35.00		as needed	7-14 days	lung, kidney, liver, nasal swab
Canine herpesvirus - CHV	Canine	VN	Virology	$5.00		F	following Monday	serum
Canine influenza virus - CIV	Canine	PCR - Subtype	Molecular	$40.00	$36.00	M, Th	next day	lung, BAL, nasal swab
Canine parvovirus - CPV	Canine	VI	Virology	$35.00		as needed	7-14 days	intestine, feces
Canine parvovirus - CPV VP2	Canine	PCR - VP2	Molecular	$65.00	$58.50	M, Th	3-5 days	Feces, intestine, fecal swab, intestinal content
Canine parvovirus - CPV VP2^	Canine	Sequencing (VP2)	Molecular	$150.00	$120.00	as needed	3-5 days	Feces, intestine, fecal swab, intestinal content	A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL.
Canine parvovirus-2 - CPV-2	Canine	PCR	Molecular	$40.00	$36.00	M, Th	next day	intestine, feces, fecal swab
Caprine arthritis encephalitis virus - CAE	Caprine, Ovine	cELISA	Serology	$6.50	$5.85	F	same day	serum
Caprine herpesvirus - CpHV	Caprine	PCR	Molecular	$40.00	$36.00	M, Th	next day	lung, nasal swab, vaginal swab, lymph node, BAL
Carbon monoxide (carboxyhemoglobin)	All Species		Analytical Chemistry Services	$40.00			5-7 days	*whole blood - EDTA (2-5mL), fetal thoracic fluid (2-5mL)
Cashe valley virus - CVV^^	Caprine, Ovine	PCR - R&D	Molecular	$20.00	$18.00	as needed	next day	placenta, fetal tissues, stomach content
CD20	Bovine, Canine, Feline, Porcine	IHC	Histopathology	$25.00		as needed		10% neutral buffered formalin-fixed tissues
CD3	Avian, Bovine, Canine, Caprine, Equine, Feline, Porcine	IHC	Histopathology	$25.00		as needed		10% neutral buffered formalin-fixed tissues
Chlamydia pecorum^^	Bovine, Caprine, Ovine	PCR - R&D	Molecular	$20.00	$18.00	as needed	next day	Joint fluid, ocular swab, feces, brain, lung, uterine swab, placenta
Chlamydia suis	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	Eye swab, vaginal swab
Cholinesterase (AChE)^^	All Species		Analytical Chemistry Services	$30.00			same day	*whole blood - EDTA (2mL), * fresh brain	Brain should be refrigerated or frozen. Severely autolyzed brain will impact results. Whole blood-EDTA should be refrigerated.
Chronic wasting disease - CWD	Ungulates	ELISA	Virology	$25.00		Th	same day	FRESH tissues:Medial retropharyngeal lymph node ANDobex	$15.00 - farm raised; $25.00 - free roaming; $12.50 - preserve
Clostridioides difficile	All Species	Culture	Bacteriology	$15.00		M-F	1 week
Clostridioides difficile	All Species	ELISA - toxin A/B	Bacteriology	$30.00		M-F	same day	colon, feces	Same day results if samples received by 2pm. Swabs are not an acceptable sample type.
Clostridioides difficile	All Species	PCR - toxin A & B genes	Molecular	$35.00	$31.50	M, Th	Next day	Feces, Fecal Swab, Intestine	This assay detects and differentiates the genes for toxin A and toxin B.
Clostridium perfringens	All Species	PCR - toxin genotyping (alpha, beta, epsilon, iota, enterotoxin, beta2)	Molecular	$30.00	$27.00	M, Th	next day	culture
Clostridium sp. ^^	All Species	FA	Bacteriology	$10.00		M-F	1-2 days	muscle
Coliform	All Species	Culture	Bacteriology	$15.00		M-F	1-2 days	water
Cortisol^^	All Species	ELISA (R&D)	Serology	$30.00	$27.00		contact section leader	serum, plasma, saliva
Coxiella burnetii (Q Fever)	Caprine, Ovine	PCR	Molecular	$30.00	$27.00	M, Th	next day	feces, milk, placenta, vaginal swab, stomach content
Coxiella burnetii - Q Fever	Bovine, Canine, Cervidae, Ovine	ELISA	Serology	$15.00	$13.50	Thursday	same day	Serum, Plasma, Milk
Cyanide^^	All Species	LC-MS	Analytical Chemistry Services	$40.00			5-7 days	whole blood-EDTA (4mL), *forage/silage/feed (50g), liver (50g), water (1 mL)	Freeze specimens promptly in airtight container and maintain frozen during shipping. A % Moisture will be included with this test for feed and forage samples.
Cyanobacterial Harmful Algal Bloom (CHAB) Toxins	All Species	LC-MS/MS	Analytical Chemistry Services	$200.00		as needed	7-10 days	water	Preferred sample size is 500mL. Panel includes anatoxin-a, cylindrospermopsin, microcystin LA, microcystin LR, microcystin RR, and microcystin YR
Cystoisospora suis	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	Next day	feces, intestine
Decalcification	All Species	Decalcification	Histopathology	$5.00		as needed
Dermatophilus Screen	All Species	Staining	Bacteriology	$30.00		M-Th	4-8 days	skin, hair, scabs, crusts	Swabs are not an acceptable sample type. This test includes culture & direct smear.
Direct Smear	All Species	Staining	Bacteriology	$10.00		M-F	same day		Gram's stain, acid fast stain
Encephalomyocarditis virus - EMCV	Porcine	VI	Virology	$35.00		as needed	7-14 days	brain, heart
Encephalomyocarditis virus - EMCV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	brain, spinal cord, heart
Epizootic hemorrhagic disease virus - EHDV	Bovine, Caprine, Cervidae, Ovine, Ungulates	PCR	Molecular	$35.00	$31.50	M, Th	next day	whole blood, spleen
Epizootic hemorrhagic disease virus and Bluetongue virus - EHDV and BTV	Bovine, Caprine, Cervidae, Ovine, Ungulates	PCR	Molecular	$60.00	$54.00	M, Th	next day	Whole-blood, spleen
Equine adenovirus 1 - EAdV1	Equine	PCR	Molecular	$45.00	$40.50	M, Th	next day	lung, nasal swab
Equine arteritis virus - EAV	Equine	PCR	Molecular	$40.00	$36.00	M-F	same day	nasal swab, EDTA blood, lung, semen, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
Equine herpesvirus type 1 - EHV1	Equine	PCR - neuropathotyping (A2254G)	Molecular	$45.00	$40.50	M-F	same day	nasal swab, lung, brain, fetal tissues, buffy coat
Equine herpesvirus type 1- EHV1	Equine	VI	Virology	$35.00		as needed	7-14 days	fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab, brain
Equine herpesvirus type 2 - EHV2	Equine	PCR	Molecular	$45.00	$40.50	M, Th	next day	nasal swab, lung
Equine herpesvirus type 3 - EHV3	Equine	PCR	Molecular	$45.00	$40.50	M, Th	Next day	Reproductive swabs
Equine herpesvirus type 3 - EHV3	Equine	VI	Virology	$35.00		as needed	7-14 days	reproductive tract scraping/swab
Equine herpesvirus type 4 - EHV4	Equine	VI	Virology	$35.00		as needed	7-14 days	fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab, brain
Equine herpesvirus type 4 - EHV4	Equine	PCR (R&D)	Molecular			M-F	same day	nasal swab, lung, brain, fetal tissues, buffy coat	CALL for pricing: 515-294-1950
Equine infectious anemia - EIA	Equine	AGID	Serology	$6.00	$5.40	M-Th	next day	serum
Equine infectious anemia - EIA	Equine	ELISA	Serology	$6.00	$5.40	M-F	same day	serum
Equine influenza virus - EIV	Equine	VI	Virology	$50.00		as needed	7-14 days	lung, nasal swab
Equine rhinitis A and B virus - ERAV & ERBV	Equine	PCR	Molecular	$45.00	$40.50	M-F	same day	nasal swab, lung
Erysipelothrix rhusiopathiae	All Species	Bacterial enrichment	Bacteriology	$20.00		M-F	8 days		$20 enrichment + routine culture fee ($15.00 - 25.00 depending on sample type).
Erysipelothrix rhusiopathiae	Porcine	FMIA Luminex	Serology	$9.00	$8.10	M	same day	serum
Erysipelothrix rhusiopathiae	All Species	IHC	Histopathology	$25.00		as needed		skin, spleen
Escherichia coli	All Species	PCR -geno typing	Molecular	$65.00	$58.50	M, Th	next day	culture	E. coli genotyping PCR targets the following genes: Toxin Genes: EAST1, LT, Sta, STb, Stx1, Stx2, Stx2e; Pilus Genes: F18, F41, K88, K99, 987P; Adhesin Genes: AIDA, EAEA, PAA
Ethylene glycol^^	All Species	GC-MS	Analytical Chemistry Services	$100.00			5-7 days	*kidney/liver (50g), *bait (50g), serum/plasma (2mL), GI contents/vomitus/feed (50g), urine (5mL)	If GI contents are submitted they need to be collected < 8 hours from the exposure.
Feed particle size^^	All Species		Analytical Chemistry Services	$20.00			3-5 days	*feed (200g)	Not for high-fat feeds or liquid feeds.
Feline calicivirus - FCV	Feline	PCR	Molecular	$40.00	$36.00	M, Th	next day	lung, nasal swab, conjunctival swab
Feline calicivirus - FCV	Feline	VI	Virology	$35.00		as needed	7-14 days	lung, nasal swab, conjunctival swab
Feline herpesvirus - FHV	Feline	PCR	Molecular	$40.00	$36.00	M, Th	next day	lung, nasal swab, conjunctival swab
Feline herpesvirus - FHV	Feline	VI	Virology	$35.00		as needed	7-14 days	lung, nasal swab, conjunctival swab
Feline panleukopenia virus - FPLV	Feline	PCR	Molecular	$40.00	$36.00	M, Th	next day	intestine, feces, fecal swab
Feline parvovirus-FPV	Feline	VI	Virology	$35.00		as needed	7-14 days	intestine, feces
Fluoride^^	All Species	ion specific electrode	Analytical Chemistry Services	$100.00			10-14 days	*serum/plasma (2mL), urine (2mL), *bone - whole bone
Fungal	All Species	Culture	Bacteriology	$25.00		M-F	20 - 30 days		$25.00 1st sample; $10.00 each additional sample
Fungal ID Culture Sent	All Species	Fungal ID	Bacteriology	$15.00		M-F	3-15 days	Agar plate or slant of pure fungal growth
GC/MS Toxicant Screen	All Species	GC-MS	Analytical Chemistry Services	$150.00		Tu	1-3 days	*GI contents (50g), feed (200g), water (100mL), serum/plasma (5mL), tissues (50g)	The gas chromatography-mass spectrometry (GC-MS) screen offers the possibility of detecting potential toxicants in a sample. Data is matched to a library of mass spectra that includes more than 200,000 compounds. The GC-MS screen is capable of detecting pesticides (organophosphorus, carbamate, pyrethroid, and chlorinated), industrial pollutants, natural products, alkaloids, vitamins, and drugs. If the specimen of interest is not listed or if you have questions regarding suspected compounds please contact the lab for further information. This test will not detect mycotoxins, heavy metals, anticoagulants, or ionophores.
Giardia^	All Species	SNAP	Parasitology	$19.00		M-F	as needed	feces
Glaesserella parasuis - GPS	Porcine	PCR - serotype	Molecular	$60.00	$54.00	M, Th	next day	Pure Culture	This assay detects serotypes 1 through 15.
Glaesserella parasuis - GPS	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	lung, BAL, bronchial swab, joint fluid, heart, fibrin, oral fluid, pleural swab
Gossypol	All Species	HPLC	Analytical Chemistry Services	$80.00			7-10 days	*feed (200g), GI contents (50g)	Assay measures the concentration of free gossypol.
Heavy Metals Panel	All Species	ICP-MS	Analytical Chemistry Services	$50.00			3-5 days	whole blood-EDTA (2mL)	Elements available: arsenic, cadmium, lead, mercury
Histopathology	All Species	IHC	Histopathology	$25.00		as needed		tissue, unstained slide
Histopathology	All Species	Slide preparation - MEGA	Histopathology	$15.00					This is an additional charge for generating a large format histology (MEGA) slide.
Histopathology	All Species	Special stains	Histopathology	$15.00		as needed		tissue, unstained slide
Iba-1	Bovine, Canine, Equine, Feline, Porcine	IHC	Histopathology	$25.00		as needed		10% neutral buffered formalin-fixed tissues
Individual Element - Tissue and Feed	All Species	ICP-MS	Analytical Chemistry Services	$25.00		M, Tu, Th, F	1-3 days	feed (200g), tissue (50g), liver biopsy	$25 per element. Elements Available: Calcium, Cadmium, Cobalt, Chromium, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Sodium, Phosphorus, Selenium, Zinc.
Individual Heavy Metal	All Species	ICP-MS	Analytical Chemistry Services	$35.00			3-5 days	whole blood-EDTA (2mL)	Elements Available: Arsenic, Cadmium, Lead, Mercury
Infectious bursal disease virus - IBDV	Avian	ELISA	Serology	$2.50	$2.25	W	same day	serum	validated for chicken only
Influenza A virus - IAV	Porcine	HI H1 - H1 gamma '99	Virology	$7.00		M	Tu	serum
Influenza A virus - IAV	Porcine	HI H3 - H3 Cluster 4	Virology	$7.00		M	Tu	serum
Influenza A virus - IAV	Canine, Equine, Feline, Porcine	PCR - Subtype only	Molecular	$45.00	$40.50	Tu, F	next day	lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab, udder wipe	For IAV subtyping added after initial screen when NOT ordered together. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available.
Influenza A virus - IAV	Porcine, Bovine	NP ELISA	Serology	$7.50	$6.75	F	same day	serum (porcine), milk (bovine), bulk tank milk (bovine)
Influenza A Virus - IAV	Porcine	HI H1N2 human-like	Virology	$7.00		M	Tu	serum
Influenza A virus - IAV	Porcine	HI Zoetis Ags (4)	Virology	$28.00		M	Tu	serum
Influenza A virus - IAV	Porcine	IHC	Histopathology	$25.00		as needed		lung
Influenza A virus - IAV	Canine, Equine, Feline, Porcine	PCR - Screen and Subtype (positives)	Molecular	$35.00	$31.50	M-F	screen - same day; subtype - next day	lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab, udder wipe	Includes subtyping all positive samples. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available.
Influenza A virus - IAV	Canine, Equine, Feline, Porcine	PCR - Screen only	Molecular	$25.00	$22.50	M-F	same day	lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab, udder wipe	Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available. Follow up IAV subtyping added on after initial testing will incur an additional fee.
Influenza A virus - IAV	Porcine	PCR - Screen & Subtype 1 USDA	Molecular	$30.00	$27.00	M-F		lung, nasal swab, oral fluids, udder wipe	Includes subtyping one positive sample per case. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available. Influenza A in Swine Testing Algorithm	Influenza A in Swine Testing Algorithm
Influenza A virus - IAV	Porcine	VI	Virology	$50.00		T, Th	7-21 days	lung, nasal swab, BAL, oral fluids
Influenza A virus - IAV	Porcine	PCR - Screen & Subtype ALL USDA	Molecular	$35.00	$31.50	M-F		lung, nasal swab, oral fluids, udder wipe	Includes subtyping of all positive samples per case. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available. Influenza A in Swine Testing Algorithm	Influenza A in Swine Testing Algorithm
Influenza A virus - IAV	Porcine	HI, H3 Cluster 1/3	Virology	$7.00		M	Tu	serum
Influenza A virus - IAV	Porcine	HI, H1 Classical '73	Virology	$7.00		M	Tu	serum
Influenza A virus - IAV	Avian, Bovine, Canine, Equine, Feline, Porcine	HI, H1 pandemic	Virology	$8.00		M	Tu	serum
Influenza A Virus - IAV	Canine, Equine, Feline, Porcine	PCR - Quantitative	Molecular	$40.00	$36.00	M-F	same day	lung, nasal swab, BAL, oral fluids, bronchial swab, oropharyngeal swab, pharyngeal swab, tracheal swab, udder wipe
Influenza A virus - IAV^	Canine, Equine, Feline, Porcine	Sequencing (hemagglutinin (HA), ~1.7kb)	Molecular	$110.00	$99.00	M, W, F	up to two business weeks from time of request	BAL, lungs, nasal swabs, oral fluid, bronchial swabs, udder wipe, and virus isolates	USDA surveillance samples may be delayed while the required isolate is obtained. Sample amount: no less than 1mL fluid, 3 grams of tissue Success rate: IAV screening PCR with a ct of 35 or lower for lung, BAL and nasal swab and a ct of 30 or lower for oral fluids is recommended. Cost: IAV HA gene PCR-$35.00 IAV Sequencing and analysis- $75.00 Total cost=$110.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL USDA-IAV-sequencing.pdf	uploads/Iowa State PPP job slides 1.2024 pdf.pdf
Influenza B virus - IBV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	lung, nasal swab
Influenza C virus - ICV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	lung, nasal swab, pharyngeal swab
Influenza D virus - IDV	Bovine, Porcine	PCR	Molecular	$30.00	$27.00	M-F	next day	Lung, nasal swab, tracheal wash, pharyngeal swab
Interpretation - Diagnostic Individual	All Species	Interpretation	Histopathology	$70.00		as needed			For clinical diagnostic submissions with gross pathology and ancillary testing where individual testing on animals from the same epidemiological unit (age, group, or lot) is requested. Cost is $70 for up to 10 slides and $7 for each additional slide.
Interpretation - Diagnostic Routine	All Species	Interpretation	Histopathology	$35.00		as needed			For clinical diagnostic submissions with gross pathology and ancillary testing where individual testing is NOT requested. Cost is $35 for up to 10 slides and $7 for each additional slide. For submissions with only histopathology, the initial fee is $45.
Interpretation - Research	All Species	Interpretation	Histopathology	$300.00		as needed			Fee is assessed per hour in 30 minute increments.
Ionophore Panel - Feed	All Species		Analytical Chemistry Services	$100.00		W	1-3 days	*corn-based feed (200g)	Panel includes: Lasalocid, Monensin, Narasin, and Salinomycin. Expected concentration required upon submission. If expected concentration is not given upon submission then additional charges may apply.
Japanese encephalitis virus - JEV^^	Equine, Porcine	PCR - R&D	Molecular	$20.00	$18.00	as needed	next day	fetal tissues
Lawsonia intracellularis (Ileitis)	Porcine	ELISA blocking	Serology	$8.00	$7.20	W	same day	serum, plasma
Lawsonia intracellularis (Ileitis)	Equine, Porcine	IHC	Histopathology	$25.00		as needed		colon, intestine
Lawsonia intracellularis (Ileitis)	Equine, Porcine	PCR	Molecular	$30.00	$27.00	M-F	same day	feces, fecal swab, intestine
Lawsonia intracellularis (Ileitis)	Equine, Porcine	PCR - Quantitative	Molecular	$35.00	$31.50	M-F	same day	feces, fecal swab, intestine
LC-MS Toxicant Screen	All Species	LC-MS	Analytical Chemistry Services	$150.00		Tu	1-3 days	feed (200g), GI contents (50g), water (100mL), serum/plasma (5mL), tissues (50g)	The liquid chromatography-mass spectrometry (LC-MS) screen offers possibility of detecting potential toxicants in a sample. Data is matched to a library containing more than 9 million spectra. The LC-MS screen is capable of detecting a wide variety of environmental contaminants and toxicants as well as drugs (antibiotics, anthelmintics, growth promoters, and ionophores). If the specimen of interest is not listed or if you have questions regarding suspected compounds, please contact the lab for further information. This test will not detect chlorinated pesticides and heavy metals.
Lead - Tissue	All Species	ICP-OES	Analytical Chemistry Services	$25.00		Tu, Th	3-5 days	kidney (50g)
Lead STAT Blood	All Species	kit	Analytical Chemistry Services	$20.00		as needed	same day	whole blood-EDTA (2mL)
Legal Case Fee	All Species		Pathology	$250.00					$250.00 for legal or insurance case processing and documentation.
Leptospira	All Species	Individual serovars	Serology	$6.00	$5.40	T, Th	same day		bratislava, canicola, copenhageni, grippotyphosa, hardjo, ictero, pomona
Leptospira	All Species	MAT 5 serovars	Serology	$5.50	$4.95	T, Th	same day	serum, fetal thoracic fluid	5 serovars = canicola, ictero, grippo, pomona, hardjo (Bison)
Leptospira	All Species	PCR	Molecular	$30.00	$27.00	M - F	same day	kidney, urine
Leptospira	All Species	MAT 6 serovars	Serology	$6.00	$5.40	T, Th	same day	serum, fetal thoracic fluid	6 serovars = canicola, ictero, grippo, pomona, hardjo, bratislava (Bison)
Leptospira hardjo bovis	Bovine	PCR	Molecular	$30.00	$27.00	M - F	same day	kidney, urine
Leptospira interrogans	All Species	IHC	Histopathology	$25.00		as needed		kidney, liver
Listeria	All Species	Enrichment culture	Bacteriology	$35.00		M-F	16 weeks		$35 enrichment + routine culture fee ($15.00 - 25.00 depending on sample type).
Listeria monocytogenes	Bovine, Caprine, Ovine, Porcine	IHC	Histopathology	$25.00		as needed		brain, placenta
Mass Spectrometry Toxicant Screen	All Species	GC-MS and LC-MS	Analytical Chemistry Services	$200.00		Tu	1-3 days	feed (200g), GI contents (50g), water (100mL), serum/plasma (5mL), tissues (50g)	Both the GC-MS and LC-MS screens offers the possibility of detecting potential toxicants in a sample. Data is matched to a library. If the specimen of interest is not listed or if you have questions regarding suspected compounds, please contact the lab for further information. Additional information is available under Laboratories > Analytical Chemistry Services > Helpful Testing Information
Methemoglobin	All Species		Analytical Chemistry Services	$35.00			7-10 days	*whole blood-EDTA (2 mL)	Samples should be chilled and submitted ASAP.
Milk Culture	All Species	Bacterial Isolation and Identification	Bacteriology	$8.00		M-F	3 - 7 days
Milk culture plus Mycoplasma	Bovine	Bacterial Isolation and Identification	Bacteriology	$10.00		M-F	3 - 14 days
Moisture (%)	All Species		Analytical Chemistry Services	$10.00			3-5 days	*feed (20g), *tissue (20g)
Mycobacterium paratuberculosis (Johne	Bovine	ELISA	Serology	$5.00	$4.50	W, F	same day	serum, plasma	Please visit https://vetmed.iastate.edu/vdpam/about/FSVM/beef/bovine-disease-topics/johnes-disease for more information.
Mycobacterium paratuberculosis (Johne's)	Bovine	CF	Serology	$6.00	$5.40	F	same day	serum
Mycobacterium paratuberculosis (Johne's)	Bovine, Caprine, Ovine	PCR	Molecular	$30.00	$27.00	M-F	same day (Up to next day)	feces, fecal swab	An extra day may be necessary as large herd surveillance cases need extra organization and oddly packaged samples (gloves/bags) require additional bio-security measures. Please visit https://vetmed.iastate.edu/vdpam/about/FSVM/beef/bovine-disease-topics/johnes-disease for more information.
Mycoplasma	Avian, Bovine, Camelid, Canine, Caprine, Cervidae, Equine, Feline, Ovine, Rodent, Ungulates	Culture	Bacteriology	$40.00		M-F	3 weeks		Suspect positives will be confirmed by MALDI-TOF-MS identification or PCR at no additional cost
Mycoplasma	Porcine	Culture	Bacteriology	$40.00		M-F	4 weeks		Suspect positives will be confirmed by PCR at no additional cost. Turn around time: 4 weeks for M. hyopneumoniae, 17 days for M. hyorhinis or M. hyosynoviae.
Mycoplasma bovis	Bovine	IHC	Histopathology	$25.00		as needed		lung
Mycoplasma bovis	Bovine	PCR	Molecular	$30.00	$27.00	M-F	same day	lung, BAL, milk, nasal swab, joint swab, tracheal wash
Mycoplasma flocculare	Porcine	PCR	Molecular	$25.00	$22.50	M, Th	next day	lung, nasal swab, culture
Mycoplasma gallisepticum - MG	Avian	ELISA	Serology	$2.50	$2.25	Tuesday	same day	serum	chicken, turkey
Mycoplasma gallisepticum - MG	Avian	PCR	Molecular	$30.00	$27.00	M-F	same day	oropharyngeal and tracheal swabs
Mycoplasma gallisepticum - MG^^	Avian	Sequencing (IGSR/mgc2/pvpA, R&D)	Molecular	$225.00	$202.50	as needed	3-5 days	Oropharyngeal swab, Tracheal swab
Mycoplasma hyopneumoniae - MHP	Porcine	ISH	Histopathology					Lung	Please contact for pricing and availability.
Mycoplasma hyopneumoniae - MHP	Porcine	ELISA	Serology	$5.50	$4.95	M-F	same day	serum, plasma
Mycoplasma hyopneumoniae - MHP	Porcine	Titration	Bacteriology	$100.00		M-F	4 weeks	Culture, challenge material	$50.00 for each additional specimen beyond the first
Mycoplasma hyopneumoniae - MHP	Porcine	IHC	Histopathology	$25.00		as needed		lung
Mycoplasma hyopneumoniae - MHP	Porcine	PCR	Molecular	$30.00	$27.00	M-F	same day	lung, bronchial swab, BAL, oral fluids, nasal swab, laryngeal swab, tracheal swab
Mycoplasma hyopneumoniae - MHP	Porcine	PCR - Quantitative	Molecular	$35.00	$31.50	M-F	same day	lung, bronchial swab, BAL, oral fluids, nasal swab, laryngeal swab, tracheal swab
Mycoplasma hyopneumoniae - MHP^	Porcine	Sequencing (P146 adhesion gene)	Molecular	$145.00	$130.50	M, W, F		lung, bronchial swab, BAL, oral fluids, nasal swab, laryngeal swab, tracheal swab	^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Mycoplasma hyopneumoniae - MHP^^	Porcine	PF ELISA	Research	$7.00				processing fluid
Mycoplasma hyorhinis - MHR	Porcine	IgG ELISA	Serology	$8.00	$7.20	W	same day	serum
Mycoplasma hyorhinis - MHR	Porcine	PCR	Molecular	$25.00	$22.50	M, Th	next day	lung, oral fluid, joint swab, joint fluid, fibrin, heart
Mycoplasma hyorhinis - MHR^^	Porcine	RNAscope®	Histopathology						Please call for pricing and availability.
Mycoplasma hyosynoviae - MHS	Porcine	PCR	Molecular	$25.00	$22.50	M, Th	next day	joint swab, joint fluid, oral fluids, lung, tonsil
Mycoplasma hyosynoviae - MHS	Porcine	MHS T20 IgG ELISA	Serology	$8.00	$7.20	W	Same day	Serum
Mycoplasma iowae - MI	Avian	PCR	Molecular	$30.00	$27.00	M-F	same day	oropharyngeal swab, tracheal swab
Mycoplasma meleagridis - MM	Avian	PCR	Molecular	$30.00	$27.00	M-F	same day	oropharyngeal swab, tracheal swab
Mycoplasma ovipneumoniae - M. ovi	Bovine, Caprine, Ovine	PCR	Molecular	$40.00	$36.00	M, Th	Next day	Nasal swab, lung
Mycoplasma spp - eMyco	All Species	PCR with Sequencing	Molecular	$100.00	$90.00	M, Th	next day if negative, one week if positive	lung, Bronchial Swab, Tracheal Swab, Ocular Swab, Cell Culture, Joint Fluid	Cost: $50 for PCR, $50 for sequencing. Total cost=$100 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Mycoplasma suis	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	whole blood (EDTA or heparin), spleen
Mycoplasma synoviae - MS	Avian	PCR	Molecular	$30.00	$27.00	M-F	same day	oropharyngeal and tracheal swabs
Mycoplasma synoviae - MS	Avian	ELISA	Serology	$2.50	$2.25	Tuesday	same day	serum	chicken, turkey
Mycoplasma synoviae^^ - MS	Avian	Sequencing (vlhA) (R&D)	Molecular	$110.00	$99.00	as needed	3-5 days	Oropharyngeal swab, Tracheal swab
Mycotoxin - Aflatoxin M1	All Species	LC-MS	Analytical Chemistry Services	$100.00			7-10 days	*liver (20g)	See the mycotoxin website page for more information.
Mycotoxins Panel	All Species	LC-MS	Analytical Chemistry Services	$100.00		Th	1-3 days	*corn, grain, feed, mixed feed (200g)	Panel includes: Aflatoxin B1, B2, G1, G2, Dexoynivalenol, 3-Acetyl Deoxynivalenol, Fumonisin B1, B2, Ochratoxin A, T-2, HT-2, Zearalenone, Zearalenol. See the mycotoxin website page for more info. Preferred specimen is corn.
Necropsy	Bovine, Camelid, Canine, Caprine, Cervidae, Equine, Feline, Ovine, Rodent, Ungulates	Necropsy - Fetal	Pathology	$10.00		as needed		Fetus/Embryo
Necropsy	Avian	Necropsy - Poultry mature (each)	Pathology	$10.00		as needed
Necropsy	All Species	Special or complex procedures	Pathology	$100.00		as needed			Fee is per hour
Necropsy	Avian	Necropsy - Poultry juvenile (each)	Pathology	$5.00		as needed
Necropsy	Avian, Bovine, Camelid, Canine, Caprine, Cervidae, Feline, Ovine, Porcine, Rodent, Ungulates	Disposal ($6 per 100 lb)	Pathology	$6.00		as needed
Necropsy	Porcine	Necropsy - Swine fetal	Pathology	$5.00		as needed
Necropsy	Equine	Disposal - Equine	Pathology	$300.00		as needed			$300 for >200# by landfill; Free for <200# by incineration This is for diagnostic cases only.
Necropsy		Necropsy - Exotic Animal	Pathology	$75.00		as needed			Other species not listed elsewhere
Necropsy		Necropsy - Fish, Bird & Rodent	Pathology	$25.00		as needed
Necropsy	Canine, Equine, Feline	Necropsy - Companion Animal	Pathology	$75.00		as needed			$75 for up to 3 animals of the same species with the same disease concern
Necropsy	Bovine, Caprine, Ovine, Porcine	Necropsy - Livestock >3	Pathology	$25.00		as needed			Fee for all animals beyond the first 3 on a case.
Necropsy	Bovine, Caprine, Ovine, Porcine	Necropsy - Livestock	Pathology	$50.00		as needed			Base fee for all Livestock species. For case including more than 3 juvenile to adult animals, additional fees may apply.
Necropsy	Avian	Necropsy - Poultry Neonate (each)	Pathology	$5.00		as needed
Neospora	All Species	IHC	Histopathology	$25.00		as needed		Brain, placenta, muscle
Neospora	Bovine	PCR	Molecular	$35.00	$31.50	M, Th	next day	Brain, liver, heart
Neospora caninum	Bovine	cELISA	Serology	$6.00	$5.40	W, F	same day	serum
Neospora caninum & Leptospira spp - Neo/Lepto	Bovine	PCR	Molecular	$35.00	$31.50	M-F	same day	Fetal tissues
Newcastle disease virus - NDV	Avian	ELISA	Serology	$2.00	$1.80	W	same day	serum
NGS^	All Species	NGS - Novel/uncharacterized pathogen detection	NextGen Sequencing	$400.00		as needed	3-4 weeks	infected clinical/body fluids, tissues, feces	$400.00/library/sample
NGS^	All Species	NGS - Mixed infection detection	NextGen Sequencing	$400.00		as needed	3-4 weeks	infected clinical/body fluids, tissues, feces	$400.00/library/sample
NGS^	All Species	NGS - Viral whole genome sequencing	NextGen Sequencing	$300.00		as needed	3-4 weeks	cell culture; infected/clinical tissue, body fluids, and feces	$300.00/cell culture isolate sample; $300.00/infected or clinical tissue sample; Ct <24
NGS^	All Species	NGS - 16s metagenomics analysis	NextGen Sequencing	$150.00		Inquiry	environmental samples, infected/clinical body fluids, tissues, and feces		Please call the lab for additional information.
NGS^	All Species	NGS - Shotgun metagenomics sequencing	NextGen Sequencing	$800.00		as needed	3-4 weeks		Provide 5-10Gb sequencing data for each sample; $800.00/sample ($650.00/sample if sample number is more than 96)
NGS^	All Species	NGS - Bacterial genomic epidemiological analysis	NextGen Sequencing	$200.00		as needed	3-4 weeks	pure bacterial strains	Identify bacterial virulome, resistome, and perform phylogenetic analysis based on maximum common genome sequences; $200.00/strain (Inquire if strain number is more than 96)
Nitrate/Nitrite	All Species	HPLC	Analytical Chemistry Services	$40.00		Th	1-3 days	*water (50mL), *ocular fluid/vitreous humor (2mL), *forage/silage (100g), GI contents (100g), serum/plasma (2mL)	Keep refrigerated. A % Moisture test will be included for all forage/silage samples.
Ornithobacterium rhinotracheale - ORT	Avian	PCR	Molecular	$30.00	$27.00	M, Th	Same day	Lungs, trachea
Ornithobacterium rhinotracheale - ORT	Avian	ELISA	Serology	$3.00	$2.70	Th	same day	serum	Chicken or turkey serum
Ovine herpesvirus type 2 - OHV-2 (Malignant Catarrhal Fever)	Bovine, Caprine, Cervidae, Ovine, Porcine	PCR	Molecular	$40.00	$36.00	M, Th	next day	nasal swab, lung, brain
Parasitology^	All Species	Baermann	Parasitology	$25.00		M-Th	next business day	feces (5 grams)	This assay requires at least 5 grams of feces and is run overnight and reported the next day. If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
Parasitology^	All Species	Sedimentation	Parasitology	$25.00		M-Th	next business day	feces (5 grams)	This assay requires at least 5 grams of feces and is run overnight and reported the next day. If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
Parasitology^	All Species	Direct Smear	Parasitology	$15.00		M-F	next business day	feces	If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
Parasitology^	All Species	Fecal flotation	Parasitology	$20.00		M-F	next business day	feces (3-5 grams)	If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
Parasitology^	All Species	McMaster floatation	Parasitology	$25.00		M-F	next business day	feces (1-2 grams)	If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
Parasitology^	All Species	Modified Wisconsin - quantitative	Parasitology	$30.00		M-F	next business day	feces	Due to the sensitivity of this test, all samples are screened first with a sugar floatation, if negative or if low numbers of oocysts are detected, the Modified Wisconsin method will then be performed for quantification. If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
Pasteurella multocida - P. mult	All Species	PCR	Molecular	$35.00	$31.50	M, Th	next day	oropharyngeal swab, nasal swab, lung
Pasteurella multocida dermonecrotic toxin - P. mult DNT	Porcine	PCR	Molecular	$35.00	$31.50	M, Th	next day	Nasal swab, culture
pH	All Species		Analytical Chemistry Services	$7.00			3-5 days	*GI contents (200g), *water (100mL)
Porcine Astrovirus type 3 - PoAstV3^^	Porcine	RNAscopeâ„¢ ISH	Histopathology					Brain, spinal cord	Please call for pricing and availability.
Porcine astrovirus type 3 - PoAstV3	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	Spinal cord, brain, feces, virus isolate
Porcine astrovirus type 4 - PoAstV4	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	Next day	Lung, nasal swab, udder wipe
Porcine circovirus type 1 - PCV1	Porcine	PCR - PCV1	Molecular	$30.00	$27.00	M-F	same day	serum, oral fluid, processing fluid
Porcine circovirus type 2 & 3 - PCV2/3	Porcine	PCR	Molecular	$35.00	$31.50	M-F	same day	serum, oral fluid, lung, lymph node, processing fluid
Porcine circovirus type 2 - PCV2	Porcine	IFA endpoint	Virology	$15.00		Th	same day	serum
Porcine circovirus type 2 - PCV2	Porcine	ELISA	Serology	$6.00	$5.40	Tu	same day	serum
Porcine circovirus type 2 - PCV2	Porcine	FFN	Virology	$16.00		M	F	serum
Porcine circovirus type 2 - PCV2	Porcine	IFA 4 dilution	Virology	$7.50		Th	same day	serum
Porcine circovirus type 2 - PCV2	Porcine	IHC	Histopathology	$25.00		as needed		kidney, lung, lymph node
Porcine circovirus type 2 - PCV2	Porcine	PCR - detects genotypes PCV2a, PCV2b, PCV2c, PCV2d, PCV2e	Molecular	$30.00	$27.00	M-F	same day	serum, oral fluid, lung, lymph node, processing fluid
Porcine circovirus type 2 - PCV2	Porcine	PCR - PCV2a/b: multiplex and differential	Molecular	$30.00	$27.00	M-F		serum, lung, lymph node, oral fluid, processing fluid
Porcine circovirus type 2 - PCV2	Porcine	PCR - Quantitative	Molecular	$35.00	$31.50	M-F	same day	serum, lung, lymph node, oral fluid, processing fluid
Porcine circovirus type 2 - PCV2	Porcine	VI	Virology	$35.00		as needed	7-14 days	serum, lung, lymph nodes, tonsil
Porcine circovirus type 2 - PCV2^	Porcine	Sequencing (ORF2)	Molecular	$150.00	$135.00	M, W, F	3-5 days	lungs, lymph nodes, oral fluid, serum, processing fluid	Sample amount: no less than 1mL fluid or 3 grams of tissue. Success rate: PCV2 PCR screening with a ct of 32 or lower is recommended. Cost: PCV ORF2 PCR - $50.00, PCV sequencing and analysis - $100.00: Total cost=$150.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Porcine circovirus type 2 IgG - PCV2	Porcine	IgG ELISA	Serology	$5.00	$4.50	Tu	same day	serum	This is a combination test - selection will give results for both IgG and IgM ELISAs with a total test price of $10
Porcine circovirus type 2 IgM - PCV2	Porcine	IgM ELISA	Serology	$5.00	$4.50	Tu	same day	serum	This is a combination test - selection will give results for both IgG and IgM ELISAs (with a total price of $10)
Porcine circovirus type 2d - PCV2d^	Porcine	PCR	Molecular	$30.00	$27.00	M-F	same day	Lung, fetal tissue, oral fluid, bronchoalveolar lavage, fecal swab, nasal swab, processing fluid
Porcine circovirus type 3 - PCV3^	Porcine	Sequencing (ORF2)	Molecular	$150.00	$135.00	M, W, F	3-5 days	lung, umbilicus, oral fluid, serum, processing fluid, fetal tissue	Sample amount: no less than 1mL fluid or 3 grams of tissue. Success rate: PCV3 PCR screening with a ct of 32 or lower is recommended. Cost: PCV ORF2 PCR - $50.00, PCV sequencing and analysis - $100.00: Total cost=$150.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Porcine circovirus type 3 - PCV3^^	Porcine	RNAscopeâ„¢ ISH	Histopathology				Call		Please call for pricing and availability.
Porcine circovirus type 3 - PCV3	Porcine	PCR	Molecular	$30.00	$27.00	M-F	same day	serum, oral fluid, lung, lymph node, processing fluid
Porcine circovirus type 3 - PCV3	Porcine	PCR - Quantitative	Molecular	$35.00	$31.50	M-F	same day	lung, lung swab, serum, blood swab, processing fluid, oral fluid
Porcine cytomegalovirus - PCMV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	nasal swab, turbinates, lung
Porcine deltacoronavirus - PDCoV^	Porcine	Sequencing, S gene	Molecular	$150.00	$135.00	M, W, F	3-5 days	Feces, fecal swab, oral fluid	Sample amount: no less than 1mL fluid or 3 grams tissue Success rate: PDCoV PCR screening with a ct of 22 or lower is recommended Cost: PDCoV S Gene PCR- $50.00, PDCoV sequencing and analysis- $100.00: Total cost=$150.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Porcine deltacoronavirus - PDCoV^	Porcine	NGS - Whole Genome Sequencing	NextGen Sequencing	$300.00		as needed		feces, fecal swabs, intestine, oral fluids
Porcine deltacoronavirus - PDCoV^^	Porcine	S1 IgG ELISA (R&D)	Serology	$7.50		F	same day	serum
Porcine deltacoronavirus -PDCoV	Porcine	PCR - Singleplex	Molecular	$25.00	$22.50	M-F	same day	feces, fecal swabs, intestine, oral fluids, environmental samples
Porcine epidemic diarrhea virus - PEDV	Porcine	S1 FMIA Luminex	Serology	$9.00	$8.10	T, Th	same day	Serum, colostrum, milk
Porcine epidemic diarrhea virus - PEDV	Porcine	PCR - non-S-INDEL vs. S-INDEL differential	Molecular	$30.00	$27.00	M-F	same day	feces, fecal swabs, intestine, oral fluids, environmental samples	This test differentiates the non-S-INDEL strain (also known as the prototype or original strain) and the S-INDEL strain (also known as the variant strain) of PEDV.
Porcine epidemic diarrhea virus - PEDV	Porcine	ISH	Histopathology					Small intestine	Contact lab for pricing and availability.
Porcine epidemic diarrhea virus - PEDV	Porcine	FFN	Virology	$20.00		M	F	serum, milk, colostrum
Porcine epidemic diarrhea virus - PEDV	Porcine	VI	Virology	$35.00		as needed		fecal swab, feces, intestine
Porcine epidemic diarrhea virus - PEDV	Porcine	S1 FMIA IgA OF	Serology	$9.00		T	same day (test cutoff 9 am for same day testing)	oral fluids
Porcine epidemic diarrhea virus - PEDV	Porcine	HTNT	Virology	$16.00		M	F	serum, milk, colostrum, and other
Porcine epidemic diarrhea virus - PEDV	Porcine	IFA screen	Virology	$5.50		M	F	serum
Porcine epidemic diarrhea virus - PEDV	Porcine	IHC	Histopathology	$25.00		as needed		intestine
Porcine epidemic diarrhea virus - PEDV	Porcine	IFA 4 dilution	Virology	$15.00		M	F	serum
Porcine epidemic diarrhea virus - PEDV	Porcine	PCR - Singleplex	Molecular	$25.00	$22.50	M-F	same day	feces, fecal swabs, intestine, oral fluids, environmental samples
Porcine epidemic diarrhea virus - PEDV	Porcine	PCR - Quantitative	Molecular	$30.00	$27.00	same day	M-F	feces, fecal swabs, intestine, oral fluids, environmental samples
Porcine epidemic diarrhea virus - PEDV^	Porcine	NGS - Whole Genome Sequencing	NextGen Sequencing	$300.00		as needed		feces, fecal swabs, intestine, oral fluids
Porcine epidemic diarrhea virus - PEDV^	Porcine	Sequencing (S1 portion, ~2.2kb)	Molecular	$150.00	$135.00	M, W, F	3-5 days	feces, fecal swab, intestines, oral fluids, environmentals	Sample amount: no less than 1mL fluid or 3 grams tissue Success rate: PEDV PCR screening with a ct of 21 or lower is recommended Cost: PEDV S1 PCR- $50.00, PEDV sequencing and analysis- $100.00: Total cost=$150.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Porcine epidemic diarrhea virus / Porcine deltacoronavirus / Transmissible gastroenteritis virus - PEDV/PDCoV/TGEV	Porcine	PCR - Multiplex	Molecular	$25.00	$22.50	M-F	same day	feces, fecal swabs, intestine, oral fluids, environmental samples
Porcine hemagglutinating encephalomyelitis virus - pHEV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	brain, spinal cord
Porcine hemagglutinating encephalomyelitis virus - pHEV^^	Porcine	IgG ELISA (R&D)	Research	$7.00		M-F	same day	serum, plasma
Porcine parainfluenza virus type 1 - PPIV^^	Porcine	ELISA (R&D)	Research	$7.50	$6.25	as requested	same day	serum
Porcine parainfluenza virus type 1 - PPIV	Porcine	PCR	Molecular	$30.00	$27.00	M-F	next day	lung, nasal swabs, bronchial swab, BAL, oral fluids
Porcine parainfluenza virus type 1 - PPIV^	Porcine	Sequencing (HN gene)	Molecular	$150.00	$135.00	M, W, F	3-5 days	lung, nasal swabs, bronchial swab, BAL, oral fluids	^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL.
Porcine parainfluenza virus type 1 - PPIV^	Porcine	Sequencing (F gene)	Molecular	$150.00	$135.00	M, W, F	3-5 days	lung, nasal swabs, oral fluids, BAL, bronchial swab	^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Porcine parvovirus - PPV	Porcine	VI	Virology	$35.00		as needed	7-14 days	aborted fetal tissue, fetal thoracic fluid
Porcine parvovirus - PPV	Porcine	HI	Virology	$7.00		M	Tu	serum
Porcine parvovirus - PPV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	fetal tissue
Porcine parvovirus type 2 - PPV2	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	serum, lung, spleen
Porcine reproductive & respiratory syndrome virus (North American and European) / Mycoplasma hyopneumoniae - PRRSV NA, PRRSV EU, MHP	Porcine	FMIA	Serology	$35.00	$31.50	Tuesday, Friday	same day	Serum
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	VI - M145 cells	Virology	$35.00		F	7-14 days	serum, oral fluid, lung
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	VI - ZMAC	Virology	$50.00		T, F	7-14 days	serum, oral fluid, lung
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	IHC	Histopathology	$25.00		as needed		lung, lymph node
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	PCR - Zoetis Fostera-like	Molecular	$35.00	$31.50	M-F	Same day	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum	This assay tests for the presence of the vaccine virus in the sample but it is not a differential assay in that it does not exclude a potential co-infection with a wild-type PRRSV.
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	PCR - Pharmgate PRRSGard MLV-like	Molecular	$35.00	$31.50	M-F	Same day	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	PCR - Elanco Prevacent-like and BIVI Ingelvac MLV-like multiplex	Molecular	$35.00	$31.50	M-F	same day	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum
Porcine reproductive & respiratory syndrome virus - PRRSV	Porcine	Zoetis Fostera MLV CLAMP Sequencing	Molecular	$220.00	$198.00	M-F	2-4 days	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum	Sample amount: no less than 1 mL fluid, 3 grams of tissue. Success rate: PRRSV screening PCR with a ct of 30 or lower is recommended. Cost: ORF5 CLAMP PCR - $90.00 Sequencing and analysis - $65 per sequence recovered, maximum of 2. Total maximum cost=$220.
Porcine reproductive & respiratory syndrome virus - PRRSV^	Porcine	Elanco Prevacent MLV CLAMP Sequencing (ORF5 region, ~600bp)	Molecular	$220.00	$198.00	M-F	2-4 days	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum	Sample amount: no less than 1 mL fluid, 3 grams of tissue. Success rate: PRRSV screening PCR with a a ct of 30 or lower is recommended. Cost: ORF5 CLAMP PCR - $90.00 Sequencing and analysis - $65 per sequence recovered, maximum of 2. Total maximum cost=$220.
Porcine reproductive & respiratory syndrome virus - PRRSV^^	Porcine	IgA ELISA (R&D)	Research	$9.00		as needed
Porcine reproductive & respiratory syndrome virus - PRRSV^^	Porcine	IgM/IgA ELISA (R&D)	Serology	$9.00		M	same day	oral fluids
Porcine reproductive & respiratory syndrome virus - PRRSV^^	Porcine	IgM ELISA (R&D)	Research	$9.00		as needed		oral fluids, serum
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	ELISA X3	Serology	$5.00	$4.50	M-F	same day	serum, plasma
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	ELISA OF	Serology	$7.50	$6.75	M-F	same day	oral fluids
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	FFN	Virology	$15.00		M	F	serum
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	IFA European -screen @ 1:20	Serology	$5.00	$4.50	M-F	same day	serum	call office
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	PCR - Quantitative	Molecular	$30.00	$27.00	M-F	same day	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	IFA European-titration	Serology	$15.00	$13.50	as needed	same day	serum
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	IFA North American -screen @ 1:20	Serology	$5.00	$4.50	M-F	same day	serum
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	IFA North American -titration	Serology	$15.00	$13.50	as needed	same day	serum
Porcine reproductive & respiratory syndrome virus- PRRSV	Porcine	PCR - Screening Type 1 (EU) and Type 2 (NA)	Molecular	$25.00	$22.50	M-F	same day	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum
Porcine reproductive & respiratory syndrome virus- PRRSV^	Porcine	Sequencing (ORF5 region, ~600bp)	Molecular	$110.00	$99.00	M-F	2-4 days	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum	Sample amount: no less than 1mL fluid or 3 grams of tissue. Success rate: PRRSV screening PCR with a ct of 35 or lower for other specimens is recommended. Cost: ORF5 PCR -$45.00 Sequencing and analysis- $65.00 Total cost=$110.00. ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL. European PRRSV PCR is tested Monday, Wednesday, and Friday with a 2-4 day turn-around time.
Porcine reproductive & respiratory syndrome virus- PRRSV^	Porcine	BIVI Ingelvac MLV CLAMP Sequencing (ORF5 region, ~600bp)	Molecular	$220.00	$198.00	M-F	2-4 days	Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum	Sample amount: no less than 1 mL fluid, 3 grams of tissue. Success rate: PRRSV screening PCR with a a ct of 30 or lower is recommended. Cost: ORF5 CLAMP PCR - $90.00 Sequencing and analysis - $65 per sequence recovered, maximum of 2. Total maximum cost=$220.
Porcine respiratory coronavirus - PRCV	Porcine	PCR	Molecular	$35.00	$31.50	M-F	same day	lung, nasal swab
Porcine Rotavirus A/B/C	Porcine	PCR	Molecular	$40.00	$36.00	Daily	Next day	Feces, intestine, fecal swab, oral fluid
Porcine sapelovirus - PSV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	spinal cord, brain, feces, fecal swab, oral fluid
Porcine sapelovirus - PSV	Porcine	VI	Virology	$35.00		as needed	7 - 14 days	serum, brain, spinal cord, lymph node
Porcine Sapovirus - PSapoV	Porcine	PCR	Molecular	$30.00	$27.00	M-F	next day	feces, fecal swab, oral fluids
Porcine Sapovirus Genogroup III - PSapoV^	Porcine	NGS - Sequencing Genogroup III VP1	NextGen Sequencing	$200.00		as needed	3-4 weeks	Feces, fecal swab	$200 will provide up to 4 gene sequences, per serotype, per sample. If more than 4 gene sequences are generated, an additional $200 will be charged.
Porcine teschovirus - PTV	Porcine	VI	Virology	$35.00		as needed	7-14 days	spinal cord, brain
Porcine teschovirus - PTV	Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	spinal cord, brain, feces, fecal swab, oral fluid	This assay detects all serotypes of PTV (1-7, 11-13).
Pseudorabies - PRV	Porcine	ELISA g1	Serology	$5.50	$4.95	as needed	same day	serum, meat juice
Pseudorabies - PRV	Bovine, Canine, Cervidae, Porcine	PCR	Molecular	$30.00	$27.00	M, Th	next day	brain, tonsil, lung, fetal tissues
Pseudorabies - PRV	Porcine	ELISA gB (screen)	Serology	$3.50	$3.15	W, F	same day	serum, meat juice
Pseudorabies - PRV	Porcine	VI	Virology	$35.00		as needed	7-14 days	brain, lung, tonsil
Pseudorabies - PRV	Porcine	VN	Virology	$5.00		F	following Monday	serum
Pullorum	Avian	AGG	Serology	$3.00	$2.70	as needed	same day	serum, whole blood
Rabbit hemorrhagic disease virus 2 - RHDV2		PCR	Molecular	$50.00	$45.00	Daily	Same day	liver	Testing must have prior approval from the State of Iowa Veterinarian.
Rabies	All Species	FA	Virology	$50.00		M-F	1-2 business days	brain
Rabies	All Species	Emergency(weekend and after-hours)	Virology	$150.00		contact lab	within 48 hours	brain
Raw Milk	Bovine, Caprine, Ovine	Standard Plate & Coliform Count	Bacteriology	$25.00		M-Th	2 days	Milk	$25 first sample, $10 each additional sample
Rotavirus group A	Bovine, Equine, Porcine	IHC	Histopathology	$25.00		as needed		intestine
Rotavirus group A	Bovine, Porcine	VI	Virology	$35.00		as needed	7-14 days	Feces, Fecal swab, intestine
Rotavirus group C	Bovine, Equine, Porcine	IHC	Histopathology	$25.00		as needed		Small intestine
Rotavirus VP4 - ROTA VP4^	Avian, Bovine, Porcine	NGS - Rotavirus VP4	NextGen Sequencing	$200.00		as needed	3-4 weeks	Feces, fecal swab	Cost is $200 for each gene and genogroup per sample. Sequencing requests require specifying the gene (VP4, VP7, or both) and the genogroup (Rota A, B or C). Mixed infections may yield more than one sequence per gene within a genogroup.
Rotavirus VP7 - ROTA VP7^	Avian, Bovine, Porcine	NGS - Rotavirus VP7	NextGen Sequencing	$200.00		as needed	3-4 weeks	Feces, fecal swab	Cost is $200 for each gene and genogroup per sample. Sequencing requests require specifying the gene (VP4, VP7, or both) and the genogroup (Rota A, B or C). Mixed infections may yield more than one sequence per gene within a genogroup.
Salmonella	All Species	PCR	Molecular	$30.00	$27.00	M-F	Next-day	Feces, intestines, culture
Salmonella	Porcine	ELISA	Serology	$8.00	$7.20	T	Same day	Serum, plasma, meatjuice
Salmonella	All Species	Check&Trace Salmonella Serotyping	Molecular	$90.00	$81.00	Tu	Next day	Pure Culture	Check&Trace Salmonella serotyping is designed for the rapid molecular confirmation and serotyping of presumptive Salmonella spp bacterial isolate. The test employs highly specific DNA markers to allow accurate identification of the Salmonella serotype when Salmonella spp. is present.
Salmonella	Bovine, Canine, Caprine, Equine, Feline, Ovine, Porcine	Broth Enrichment	Bacteriology	$10.00		M-F	1-3 days		$10 enrichment + routine culture fee ($15.00 - 25.00 depending on sample type). If enriching for PCR, $10 enrichment + Salmonella PCR fee ($30.00). If PCR positive, sample will be cultured and $25 routine culture fee will be added.
Salmonella	Bovine, Canine, Caprine, Equine, Feline, Ovine, Porcine	Bacterial serogrouping	Bacteriology	$20.00		M-F	1-2 days		This fee is added whenever a Salmonella species is isolated and serogrouped. Isolate may be sent to NVSL for serotyping at no additional cost.
Salmonella (All types)	Avian	Culture - All types	Bacteriology	$30.00	$27.00	M-F	minimum 6 days	Chick Papers, DOA (Viscera), Feces, Feed
Salmonella (S. dublin)	Bovine	ELISA	Serology	$6.00	$5.40	M	same day	serum, plasma, milk, colostrum
Salmonella (SE only)	Avian	Strip Test	Bacteriology	$30.00	$27.00	M-F	minimum 3 days	Environmentals, Chick paper swabs, Chick papers, Eggs	Egg testing days are M-W.
Salmonella (SH)	Avian	Culture - SH	Bacteriology	$15.00		M-F	minimum 5 days	Environmentals, Chick paper swabs, Chick papers, Eggs	This is an add-on fee who request both SE and SH testing.
Senecavirus A - SVA	Porcine	VI	Virology	$35.00		as needed	7-14 days	serum, tissue (vesicles), vesicular fluid, oral fluid
Senecavirus A - SVA	Porcine	IFA Screen	Virology	$9.00		M	F	Serum
Senecavirus A - SVA	Porcine	4-dil IFA	Virology	$15.00		M	F	serum
Senecavirus A - SVA	Porcine	PCR	Molecular	$30.00	$27.00	M-F	same day	serum, tissue (vesicles), oral fluid, vesicular fluid and vesicular swabs	Contact your state health official first if lesions suggest a foreign animal disease. Appropriate swabs to use for SVA PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available.
Senecavirus A - SVA^	Porcine	Sequencing (VP1)	Molecular	$150.00	$135.00	as needed	3-5 days	serum, tissue (vesicles), vesicular fluid, vesicular swab, oral fluid	PCR - SVA VP1 = $50; Sequencing and Analysis = $100 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
Slide preparation with existing block	All Species	Slide preparation	Histopathology	$5.50		as needed			Per slide from existing paraffin block & return only
Slide preparation with interpretation	All Species	Slide preparation	Histopathology	$10.00		as needed			Per slide on cases with histopathologic interpretation at ISU VDL
Slide preparation without interpretation	All Species	Slide preparation	Histopathology	$15.00		as needed
Small Ruminant Abortion Panel - SRAP	Caprine, Ovine	PCR	Molecular	$65.00	$58.50	M, Th	Same day	Placenta, fetal tissues, stomach content	This assay includes Toxoplasma gondii, Campylobacter jejuni, Campylobacter fetus, Coxiella burnetii, Chlamydia abortus and Caprine herpesvirus.
Special Projects - R&D^^	All Species	ELISA, other	Serology	$0.00		as needed	test dependent	Serum, oral fluids, milk, colostrum	Specimen, turn around time and price are project dependent. Contact Serology section leader for further information.
Standard Plate Count with ID	All Species	Bacterial quantification with ID of one organism	Bacteriology	$35.00		M-Th	1-7 days	Swabs are not acceptable sample type; minimum of 1g of sample	Fee is per sample. This test quantifies the total number of bacteria present by serial dilutions with identification of one organism. Results are reported in scientific notation. If more than ten samples are submitted, contact the laboratory in advance.
Streptococcus suis - S. suis	Porcine	Bacterial serotyping	Bacteriology	$20.00		M-F	1-2 days		Serotyping is done via agglutination. Turn around time: 2-4 days if isolation required.
Streptococcus suis^^	Porcine	PCR - General and mrp virulence associated gene	Molecular	$35.00	$31.50	M, Th	next day	isolate/culture , brain, fibrin, heart, joint fluid, lung
Sulfur	All Species	ICP-OES	Analytical Chemistry Services	$30.00		Th	1-3 days	*water (100mL), *feed (100g)	STAT fees may apply.
Theileria orientalis	Bovine, Caprine, Equine, Ovine	PCR	Molecular	$35.00	$31.50	M, Th	next day	Whole blood, spleen
Toxic Element Panel	All Species	ICP-OES	Analytical Chemistry Services	$35.00		Tu, Th	3-5 days	*water (100mL), *feed (200g), *tissue (50g)	Panel includes: Antimony, Arsenic, Barium, Boron, Cadmium, Calcium, Chromium, Cobalt, Copper, Iron, Lead, Magnesium, Manganese, Mercury, Molybdenum, Phosphorus, Potassium, Selenium, Sodium, Thallium, Zinc. This is NOT recommended for evaluating the nutritional status of an animal. Reference ranges are not species specific.
Toxoplasma gondii	All Species	ELISA	Serology	$25.00	$22.50	F	same day	serum
Toxoplasma gondii	All Species	IHC	Histopathology	$25.00		as needed		brain, muscle, placenta
Toxoplasma gondii	Bovine, Caprine, Feline, Ovine	PCR	Molecular	$30.00	$27.00	Daily	Same day	Placenta, fetal tissues, stomach content, brain, feces
Mineral Panel - Serum, Plasma, and Water	All Species	ICP-MS	Analytical Chemistry Services	$50.00		M, Tu, Th, F	1-3 days	*serum/plasma (2mL), *water (100mL)	Panel includes 10 minerals: Calcium, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Phosphorus, Selenium, Zinc.
Mineral Panel - Tissue and Feed	All Species	ICP-MS	Analytical Chemistry Services	$55.00		M, Tu, Th, F	1-3 days	*feed (200g), *tissue (50g), liver biopsy (100mg)	Panel includes 14 minerals: Calcium, Cadmium, Cobalt, Chromium, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Sodium, Phosphorus, Selenium, Zinc. Liver biopsy samples should be refrigerated or frozen.
Transmissible gastroenteritis virus - TGEV	Porcine	ELISA	Serology	$8.00	$7.20	Th	same day
Transmissible gastroenteritis virus - TGEV	Porcine	IHC	Histopathology	$25.00		as needed		intestine
Transmissible gastroenteritis virus - TGEV	Porcine	PCR	Molecular	$25.00	$22.50	M-F	same day	feces, fecal swab, intestine, environmental, oral fluid
Transmissible gastroenteritis virus - TGEV	Porcine	VN	Virology	$5.00		F	following Wednesday	serum
Transmissible gastroenteritis virus / Porcine respiratory coronavirus - TGEV/PRCV	Porcine	ELISA differential	Serology	$9.50	$8.55	Th	same day	serum
Trichomonas	Bovine	Bacterial culture	Bacteriology	$10.00		M-F	1 week	trich pouch	Samples should arrive at the laboratory on Monday-Friday during business hours within 24-48 hours of collection for test validity. Refrigerated or frozen samples are not suitable for culture and will be tested by PCR.
Tritrichomonas foetus	Bovine, Feline	PCR	Molecular	$30.00	$27.00	M-F	same day	inoculated trich pouch (InPouch), preputial wash, feline-feces only		Sample Collection Instructions
Urea	All Species	HPLC	Analytical Chemistry Services	$40.00			5-7 days	*feed/fertilizer (100g), *water (100 mL)	All specimens should be frozen and maintained frozen during shipping.
Ureaplasma	Bovine	PCR	Molecular	$35.00	$31.50	M, Th	next day	lung, placenta, vulvovaginal swab
Vesicular stomatitis - VS	Bovine, Camelid, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates	VN	Virology	$5.00		F	following Monday	serum	Indiana or New Jersey Serotype (please specify) (Bison)
Virus growth	All Species	Virus propagation	Virology	$30.00		contact lab
Virus isolation shipping		VI shipping	Virology	$50.00
Virus quantitation	All Species	Virus quantitation	Virology	$120.00		contact lab	contact lab
Vitamin D - Serum	All Species	LC-MS	Analytical Chemistry Services	$40.00		M	1-3 days	*serum (0.5mL)	Test is for the 25 hydroxy form of Vitamin D3.
Vitamin D - Tissue and Feed^	All Species	Referral laboratory	Analytical Chemistry Services	$300.00			7-10 days	*feed (200g), liver (50g)	Testing referred to accredited laboratory.
Vitamins A or E - Feed	All Species	HPLC	Analytical Chemistry Services	$100.00		Tu, F	3-5 days	*corn-based feed, grain, mixed feed (200g)	$100 per analyte. Can analyze feed samples for Vitamin A Acetate, A Palmitate, or E Acetate. Expected concentration required upon submission.
Vitamins A or E - Liver	All Species	HPLC	Analytical Chemistry Services	$32.00		Tu, F	3-5 days	*liver (100g)
Vitamins A or E - Serum	All Species	HPLC	Analytical Chemistry Services	$25.00		Tu, F	3-5 days	*serum (2mL)
Water Quality Panel	All Species		Analytical Chemistry Services	$40.00		Th	1-3 days	*water (500mL)	For livestock suitability. Panel includes: Nitrate, Total Dissolved Solids, Sulfur, Coliform bacteria. Samples should be submitted in clean unused bottle. If submitting samples in a choliform bottle then an additional 400 mL of sample is needed in a separate clean bottle for the rest of the testing to be completed
West nile virus - WNV	All Species	IHC	Histopathology	$25.00		as needed		brain
West nile virus - WNV	Equine	IgM ELISA	Serology	$45.00	$40.50	M	next day	serum
West nile virus - WNV	Avian, Equine	PCR	Molecular	$40.00	$36.00	M, Th	next day	brain, serum	WNV PCR based on species: 1) Mosquitoes: mosquito pools 2) Birds: Serum and bird tissues 3) Horses: Preferable brain and spinal cord; Cerebrospinal fluid is acceptable
	All Species	RNAscope®	Histopathology						Please call for pricing and availability.
Test A
Test 1
Test 4
Influenza A virus - IAV^	Canine, Equine, Feline, Porcine	Sequencing (hemagglutinin (HA), ~1.7kb)	Molecular	$110.00	$99.00	M, W, F	up to two business weeks from time of request	BAL, lungs, nasal swabs, oral fluid, bronchial swabs, udder wipe, and virus isolates	USDA surveillance samples may be delayed while the required isolate is obtained. Sample amount: no less than 1mL fluid, 3 grams of tissue Success rate: IAV screening PCR with a ct of 35 or lower for lung, BAL and nasal swab and a ct of 30 or lower for oral fluids is recommended. Cost: IAV HA gene PCR-$35.00 IAV Sequencing and analysis- $75.00 Total cost=$110.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL USDA-IAV-sequencing.pdf	USDA-IAV-sequencing.pdf
Listeria monocytogenes & ivanovii	All Species	PCR - multiplex	Molecular	$35.00	$31.50	M, Th	Next day	brain, lung, placenta, liver
Bovine Influenza A virus - BoIAV	Bovine	PCR	Molecular	$30.00	$27.00	M-F	Same day	Milk
Bovine Influenza A virus - BoIAV	Bovine	PCR - H5	Molecular	$20.00	$18.00	M-F	Same day	Milk
Avian Influenza virus - AIV	Avian	PCR - H7	Molecular	$30.00	$27.00	M-F	Same day	cloacal swab, oropharyngeal swab, tissue samples
Bovine Coronavirus - BCoV	Bovine	ELISA	Serology	$12.00	$10.80	Wednesday	same day	Serum, plasma, milk
Micro Mineral Panel - Liver	All species	ICP-MS	Analytical Chemistry Services	$55.00		M, Tu, Th, F	1-3 days	Liver (50g), liver biopsy (100mg)	Panel includes: cobalt, copper, iron, manganese, molybdenum, selenium, and zinc. Samples should be refrigerated or frozen.
Tremorgen Panel	All species	GC-MS and LC-MS	Analytical Chemistry	N/A		Tu	1-3 days	GI content, feed (200g)	Clients are encouraged to use the Mass Spectrometry Toxicant Screen when exposure to tremorgenic mycotoxins is the suspected cause of death. Please note that tremorgenic mycotoxins are a concern in the case history and contact the lab prior to sample submission.